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4 hne antibody  (Bioss)


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    Bioss 4 hne antibody
    GPX4 and SLC7A11 expressions decreased in impaired astrocytes of ME7-infected mice, <t>meanwhile,</t> <t>4-HNE</t> increased. (A-B) Representative immunofluorescence image and relative fluorescence intensity of GPX4 expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing GPX4 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (C-D) Representative immunofluorescence images and relative fluorescence intensity of SLC7A11 expression in the thalamus region of brains from ME7-infected mice or non-infected controls (CTL), showing SLC7A11 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (E-F) Representative immunofluorescence images and relative fluorescence intensity of 4-HNE protein expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing 4-HNE (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (G) Representative western blots showing GPX4, SLC7A11, and 4-HNE levels in the brain homogenates of ME7-infected and control mice. (H) Quantification of the immunoblots in (G). (I) Immunofluorescence image showing GPX4 (green) and NeuN (red) expression in the thalamus region of ME7-infected mice or non-infected controls (CTL). Scale bar = 50 μm. (J-K) Relative fluorescence intensity of NeuN and GPX4 compared to controls. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student's two-tailed t-test GPX4: Glutathione peroxidase 4, SLC7A11: Solute carrier family 7 member 11, 4-HNE: 4-Hydroxynonenal, NeuN: Neuronal nuclei, GPX4: Glutathione peroxidase 4.
    4 Hne Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/4+hydroxynonenal+polyclonal+antibody/4+Hydroxynonenal+Polyclonal+Antibody/pmc13090729-100-33-36
    Average 96 stars, based on 295 article reviews
    4 hne antibody - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Ferroptosis-related mechanisms in prion diseases provide insights into neurodegeneration and reveal therapeutic implications"

    Article Title: Ferroptosis-related mechanisms in prion diseases provide insights into neurodegeneration and reveal therapeutic implications

    Journal: Redox Biology

    doi: 10.1016/j.redox.2026.104155

    GPX4 and SLC7A11 expressions decreased in impaired astrocytes of ME7-infected mice, meanwhile, 4-HNE increased. (A-B) Representative immunofluorescence image and relative fluorescence intensity of GPX4 expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing GPX4 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (C-D) Representative immunofluorescence images and relative fluorescence intensity of SLC7A11 expression in the thalamus region of brains from ME7-infected mice or non-infected controls (CTL), showing SLC7A11 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (E-F) Representative immunofluorescence images and relative fluorescence intensity of 4-HNE protein expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing 4-HNE (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (G) Representative western blots showing GPX4, SLC7A11, and 4-HNE levels in the brain homogenates of ME7-infected and control mice. (H) Quantification of the immunoblots in (G). (I) Immunofluorescence image showing GPX4 (green) and NeuN (red) expression in the thalamus region of ME7-infected mice or non-infected controls (CTL). Scale bar = 50 μm. (J-K) Relative fluorescence intensity of NeuN and GPX4 compared to controls. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student's two-tailed t-test GPX4: Glutathione peroxidase 4, SLC7A11: Solute carrier family 7 member 11, 4-HNE: 4-Hydroxynonenal, NeuN: Neuronal nuclei, GPX4: Glutathione peroxidase 4.
    Figure Legend Snippet: GPX4 and SLC7A11 expressions decreased in impaired astrocytes of ME7-infected mice, meanwhile, 4-HNE increased. (A-B) Representative immunofluorescence image and relative fluorescence intensity of GPX4 expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing GPX4 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (C-D) Representative immunofluorescence images and relative fluorescence intensity of SLC7A11 expression in the thalamus region of brains from ME7-infected mice or non-infected controls (CTL), showing SLC7A11 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (E-F) Representative immunofluorescence images and relative fluorescence intensity of 4-HNE protein expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing 4-HNE (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (G) Representative western blots showing GPX4, SLC7A11, and 4-HNE levels in the brain homogenates of ME7-infected and control mice. (H) Quantification of the immunoblots in (G). (I) Immunofluorescence image showing GPX4 (green) and NeuN (red) expression in the thalamus region of ME7-infected mice or non-infected controls (CTL). Scale bar = 50 μm. (J-K) Relative fluorescence intensity of NeuN and GPX4 compared to controls. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student's two-tailed t-test GPX4: Glutathione peroxidase 4, SLC7A11: Solute carrier family 7 member 11, 4-HNE: 4-Hydroxynonenal, NeuN: Neuronal nuclei, GPX4: Glutathione peroxidase 4.

    Techniques Used: Infection, Immunofluorescence, Fluorescence, Expressing, Marker, Staining, Western Blot, Control, Two Tailed Test

    Related Articles

    Immunoprecipitation:

    Article Title: Nanoimiquimod for oral cancer prevention.
    Article Snippet: ehydration, the sections were incubated with primary antibodies against NFKB (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, NFkB p65 Polyclonal Antibody # PA5-27617; dilution 1:100) and HNE (Bioss, Thermo Fisher Scientific, Waltham, MA, USA, 4 Hydroxynonenal Polyclonal Antibody #BS-6313R; dilution 1:100). After a 12-hour incubation period, the sections were washed in PBS and then incubate

    FLAG-tag:

    Article Title: Nanoimiquimod for oral cancer prevention.
    Article Snippet: ehydration, the sections were incubated with primary antibodies against NFKB (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, NFkB p65 Polyclonal Antibody # PA5-27617; dilution 1:100) and HNE (Bioss, Thermo Fisher Scientific, Waltham, MA, USA, 4 Hydroxynonenal Polyclonal Antibody #BS-6313R; dilution 1:100). After a 12-hour incubation period, the sections were washed in PBS and then incubate

    Immunofluorescence:

    Article Title: Nanoimiquimod for oral cancer prevention.
    Article Snippet: ehydration, the sections were incubated with primary antibodies against NFKB (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, NFkB p65 Polyclonal Antibody # PA5-27617; dilution 1:100) and HNE (Bioss, Thermo Fisher Scientific, Waltham, MA, USA, 4 Hydroxynonenal Polyclonal Antibody #BS-6313R; dilution 1:100). After a 12-hour incubation period, the sections were washed in PBS and then incubate

    Immunohistochemistry:

    Article Title: Nanoimiquimod for oral cancer prevention.
    Article Snippet: ehydration, the sections were incubated with primary antibodies against NFKB (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, NFkB p65 Polyclonal Antibody # PA5-27617; dilution 1:100) and HNE (Bioss, Thermo Fisher Scientific, Waltham, MA, USA, 4 Hydroxynonenal Polyclonal Antibody #BS-6313R; dilution 1:100). After a 12-hour incubation period, the sections were washed in PBS and then incubate

    Blocking Assay:

    Article Title: Nanoimiquimod for oral cancer prevention.
    Article Snippet: ehydration, the sections were incubated with primary antibodies against NFKB (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, NFkB p65 Polyclonal Antibody # PA5-27617; dilution 1:100) and HNE (Bioss, Thermo Fisher Scientific, Waltham, MA, USA, 4 Hydroxynonenal Polyclonal Antibody #BS-6313R; dilution 1:100). After a 12-hour incubation period, the sections were washed in PBS and then incubate

    Incubation:

    Article Title: Nanoimiquimod for oral cancer prevention.
    Article Snippet: ehydration, the sections were incubated with primary antibodies against NFKB (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, NFkB p65 Polyclonal Antibody # PA5-27617; dilution 1:100) and HNE (Bioss, Thermo Fisher Scientific, Waltham, MA, USA, 4 Hydroxynonenal Polyclonal Antibody #BS-6313R; dilution 1:100). After a 12-hour incubation period, the sections were washed in PBS and then incubate

    Milk:

    Article Title: Nanoimiquimod for oral cancer prevention.
    Article Snippet: ehydration, the sections were incubated with primary antibodies against NFKB (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, NFkB p65 Polyclonal Antibody # PA5-27617; dilution 1:100) and HNE (Bioss, Thermo Fisher Scientific, Waltham, MA, USA, 4 Hydroxynonenal Polyclonal Antibody #BS-6313R; dilution 1:100). After a 12-hour incubation period, the sections were washed in PBS and then incubate



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    Effect of ketogenic diet intervention on oxidative stress and neuroinflammation in aged rats. (A) Representative immunofluorescence images showing β -III tubulin for neurons (red), Iba1 for microglia (cyan), GFAP for astrocytes (blue), and <t>4HNE</t> (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fifth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. All images were acquired at 20X magnification and 3×2 tiles. Graphs showing the average intensity of 4HNE in neurons (B) , microglia (C) , and astrocytes (D) , respectively, quantified using the Ilastik-QuPath workflow for calculating the mean 4HNE intensity in each cell type expressed as arbitrary units (A.U.). Graphs showing the integrated density (Int. Den.) per mm 2 expressed as arbitrary units (A.U.) (E) and no. of 4HNE puncta per mm 2 (F) quantified using NIH-ImageJ software. (G) Representative immunofluorescence images showing β-III tubulin (red), Iba1 (cyan), and TREM2 (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fourth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. Graphs of mean TREM2 intensity in neurons (H) and microglia (I) , respectively quantified using the Ilastik-QuPath workflow for quantification of mean TREM2 intensity in each cell type expressed as A.U. (J) TREM2 integrated density per mm2 expressed as arbitrary units (A.U.) quantified using NIH-ImageJ software. Individual data points represent data from the CA1 region of the hippocampus from one brain tissue section; three brain sections were imaged per rat and approximately 100–200 cells were analyzed per cell type. The number of detections for each individual cell type for each marker is given in , and the number of rats used for the analysis are given in . Data represent Mean ± SEM. Mixed-effects two-way ANOVA (sex × diet) with animal as a random factor (fields nested within animals) and Tukey post hoc test. * p < 00.05, ** p < 0.01. Control diet (CD) and ketogenic diet (KD).
    Rabbit Anti 4 Hydroxynonenal 4hne, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of ketogenic diet intervention on oxidative stress and neuroinflammation in aged rats. (A) Representative immunofluorescence images showing β -III tubulin for neurons (red), Iba1 for microglia (cyan), GFAP for astrocytes (blue), and <t>4HNE</t> (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fifth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. All images were acquired at 20X magnification and 3×2 tiles. Graphs showing the average intensity of 4HNE in neurons (B) , microglia (C) , and astrocytes (D) , respectively, quantified using the Ilastik-QuPath workflow for calculating the mean 4HNE intensity in each cell type expressed as arbitrary units (A.U.). Graphs showing the integrated density (Int. Den.) per mm 2 expressed as arbitrary units (A.U.) (E) and no. of 4HNE puncta per mm 2 (F) quantified using NIH-ImageJ software. (G) Representative immunofluorescence images showing β-III tubulin (red), Iba1 (cyan), and TREM2 (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fourth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. Graphs of mean TREM2 intensity in neurons (H) and microglia (I) , respectively quantified using the Ilastik-QuPath workflow for quantification of mean TREM2 intensity in each cell type expressed as A.U. (J) TREM2 integrated density per mm2 expressed as arbitrary units (A.U.) quantified using NIH-ImageJ software. Individual data points represent data from the CA1 region of the hippocampus from one brain tissue section; three brain sections were imaged per rat and approximately 100–200 cells were analyzed per cell type. The number of detections for each individual cell type for each marker is given in , and the number of rats used for the analysis are given in . Data represent Mean ± SEM. Mixed-effects two-way ANOVA (sex × diet) with animal as a random factor (fields nested within animals) and Tukey post hoc test. * p < 00.05, ** p < 0.01. Control diet (CD) and ketogenic diet (KD).
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    Image Search Results


    GPX4 and SLC7A11 expressions decreased in impaired astrocytes of ME7-infected mice, meanwhile, 4-HNE increased. (A-B) Representative immunofluorescence image and relative fluorescence intensity of GPX4 expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing GPX4 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (C-D) Representative immunofluorescence images and relative fluorescence intensity of SLC7A11 expression in the thalamus region of brains from ME7-infected mice or non-infected controls (CTL), showing SLC7A11 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (E-F) Representative immunofluorescence images and relative fluorescence intensity of 4-HNE protein expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing 4-HNE (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (G) Representative western blots showing GPX4, SLC7A11, and 4-HNE levels in the brain homogenates of ME7-infected and control mice. (H) Quantification of the immunoblots in (G). (I) Immunofluorescence image showing GPX4 (green) and NeuN (red) expression in the thalamus region of ME7-infected mice or non-infected controls (CTL). Scale bar = 50 μm. (J-K) Relative fluorescence intensity of NeuN and GPX4 compared to controls. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student's two-tailed t-test GPX4: Glutathione peroxidase 4, SLC7A11: Solute carrier family 7 member 11, 4-HNE: 4-Hydroxynonenal, NeuN: Neuronal nuclei, GPX4: Glutathione peroxidase 4.

    Journal: Redox Biology

    Article Title: Ferroptosis-related mechanisms in prion diseases provide insights into neurodegeneration and reveal therapeutic implications

    doi: 10.1016/j.redox.2026.104155

    Figure Lengend Snippet: GPX4 and SLC7A11 expressions decreased in impaired astrocytes of ME7-infected mice, meanwhile, 4-HNE increased. (A-B) Representative immunofluorescence image and relative fluorescence intensity of GPX4 expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing GPX4 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (C-D) Representative immunofluorescence images and relative fluorescence intensity of SLC7A11 expression in the thalamus region of brains from ME7-infected mice or non-infected controls (CTL), showing SLC7A11 (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (E-F) Representative immunofluorescence images and relative fluorescence intensity of 4-HNE protein expression in the thalamus region of ME7-infected mice or non-infected controls (CTL), showing 4-HNE (green) in astrocytes expressing the marker GFAP (red) (n = 3 mice per group). Nuclei stained with DAPI are shown in blue. Scale bar = 50 μm. (G) Representative western blots showing GPX4, SLC7A11, and 4-HNE levels in the brain homogenates of ME7-infected and control mice. (H) Quantification of the immunoblots in (G). (I) Immunofluorescence image showing GPX4 (green) and NeuN (red) expression in the thalamus region of ME7-infected mice or non-infected controls (CTL). Scale bar = 50 μm. (J-K) Relative fluorescence intensity of NeuN and GPX4 compared to controls. Data are presented as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student's two-tailed t-test GPX4: Glutathione peroxidase 4, SLC7A11: Solute carrier family 7 member 11, 4-HNE: 4-Hydroxynonenal, NeuN: Neuronal nuclei, GPX4: Glutathione peroxidase 4.

    Article Snippet: The sections were incubated overnight at 4 °C in a humidity chamber with glial fibrillary acidic protein (GFAP, 1:50, Santa Cruz Biotechnology, sc-33673), GPX4 (1:50, Santa Cruz Biotechnology, sc-166570), SLC7A11 (1:100, ABclonal, A13685), 4-HNE antibody (1:50, Bioss Inc., bs-6313R), and NeuN (1:50, Abcam, ab177487) overnight in a humidity chamber at 4 °C.

    Techniques: Infection, Immunofluorescence, Fluorescence, Expressing, Marker, Staining, Western Blot, Control, Two Tailed Test

    Effect of ketogenic diet intervention on oxidative stress and neuroinflammation in aged rats. (A) Representative immunofluorescence images showing β -III tubulin for neurons (red), Iba1 for microglia (cyan), GFAP for astrocytes (blue), and 4HNE (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fifth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. All images were acquired at 20X magnification and 3×2 tiles. Graphs showing the average intensity of 4HNE in neurons (B) , microglia (C) , and astrocytes (D) , respectively, quantified using the Ilastik-QuPath workflow for calculating the mean 4HNE intensity in each cell type expressed as arbitrary units (A.U.). Graphs showing the integrated density (Int. Den.) per mm 2 expressed as arbitrary units (A.U.) (E) and no. of 4HNE puncta per mm 2 (F) quantified using NIH-ImageJ software. (G) Representative immunofluorescence images showing β-III tubulin (red), Iba1 (cyan), and TREM2 (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fourth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. Graphs of mean TREM2 intensity in neurons (H) and microglia (I) , respectively quantified using the Ilastik-QuPath workflow for quantification of mean TREM2 intensity in each cell type expressed as A.U. (J) TREM2 integrated density per mm2 expressed as arbitrary units (A.U.) quantified using NIH-ImageJ software. Individual data points represent data from the CA1 region of the hippocampus from one brain tissue section; three brain sections were imaged per rat and approximately 100–200 cells were analyzed per cell type. The number of detections for each individual cell type for each marker is given in , and the number of rats used for the analysis are given in . Data represent Mean ± SEM. Mixed-effects two-way ANOVA (sex × diet) with animal as a random factor (fields nested within animals) and Tukey post hoc test. * p < 00.05, ** p < 0.01. Control diet (CD) and ketogenic diet (KD).

    Journal: Frontiers in Aging Neuroscience

    Article Title: Ketogenic interventions enhance REM sleep in females and support memory in aged rats

    doi: 10.3389/fnagi.2026.1797686

    Figure Lengend Snippet: Effect of ketogenic diet intervention on oxidative stress and neuroinflammation in aged rats. (A) Representative immunofluorescence images showing β -III tubulin for neurons (red), Iba1 for microglia (cyan), GFAP for astrocytes (blue), and 4HNE (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fifth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. All images were acquired at 20X magnification and 3×2 tiles. Graphs showing the average intensity of 4HNE in neurons (B) , microglia (C) , and astrocytes (D) , respectively, quantified using the Ilastik-QuPath workflow for calculating the mean 4HNE intensity in each cell type expressed as arbitrary units (A.U.). Graphs showing the integrated density (Int. Den.) per mm 2 expressed as arbitrary units (A.U.) (E) and no. of 4HNE puncta per mm 2 (F) quantified using NIH-ImageJ software. (G) Representative immunofluorescence images showing β-III tubulin (red), Iba1 (cyan), and TREM2 (green) of the hippocampal region from coronal brain sections (50 μm) of aged male and female rats. The fourth column shows an enlarged merged image of the CA1 region of the hippocampus for the indicated groups. Graphs of mean TREM2 intensity in neurons (H) and microglia (I) , respectively quantified using the Ilastik-QuPath workflow for quantification of mean TREM2 intensity in each cell type expressed as A.U. (J) TREM2 integrated density per mm2 expressed as arbitrary units (A.U.) quantified using NIH-ImageJ software. Individual data points represent data from the CA1 region of the hippocampus from one brain tissue section; three brain sections were imaged per rat and approximately 100–200 cells were analyzed per cell type. The number of detections for each individual cell type for each marker is given in , and the number of rats used for the analysis are given in . Data represent Mean ± SEM. Mixed-effects two-way ANOVA (sex × diet) with animal as a random factor (fields nested within animals) and Tukey post hoc test. * p < 00.05, ** p < 0.01. Control diet (CD) and ketogenic diet (KD).

    Article Snippet: The following primary antibodies were used: rabbit anti- 4-hydroxynonenal (4HNE) (1:500; Bioss bs-6313R), rabbit anti- 3-nitrotyrosine (3-NT) (1:200; Bioss BS-8551R), rabbit anti- triggering receptor expressed on myeloid cells 2 (TREM2) (1:200; Bioss BS-2723R), rabbit anti- NLR family pyrin domain containing 3 (NLRP3) (1:100; NBP2-12446), mouse anti-beta- III tubulin (1:400; Abcam AB78078), rat anti- ionized calcium-binding adaptor molecule 1 (IBA) (1:1000; Abcam AB283346 ), chicken anti- glial fibrillary acidic protein (GFAP) (1:1000; Abcam AB4674).

    Techniques: Immunofluorescence, Software, Marker, Control